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ELISAGenie/Human KIM-1 (Kidney Injury Molecule 1) ELISA Kit (HUES01453)/Kidney Injury Molecule 1) ELISA Kit (HUES01453/HUES01453
ELISAGenie/Human KIM-1 (Kidney Injury Molecule 1) ELISA Kit (HUES01453)/Kidney Injury Molecule 1) ELISA Kit (HUES01453/HUES01453
- 产品编号： HUES01453
- 美 元 价： $499.00
- 市 场 价： ¥9980.00
- 品 牌： ELISAGenie
- 产 地： 美国(厂家直采)
- 公 司： ELISAGenie
- 产品分类： 蛋白类>多肽>多肽合成>
- 公司分类：
- 联系 Q Q: 1570468124
- 电话号码: 4000-520-616
- 邮 箱: info@ebiomall.com
- Description
- Additional Information
Description
Human KIM-1 (Kidney Injury Molecule 1) ELISA Kit
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KIM-1 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KIM-1. The concentration of Human KIM-1 in samples can be calculated by comparing the OD of the samples to the standard curve.
| Assay type | Sandwich |
| Format | 96T |
| Assay time | 4.5h |
| Reactivity | Human |
| Detection Method | Colormetric |
| Detection Range | 0.16-10 ng/mL |
| Sensitivity | 0.10 ng/mL |
| Sample Volume Required Per Well | 100uL |
| Sample Type | Serum, plasma and other biological fluids |
Specificity
This kit recognizes Human KIM-1in samples. No significant cross-reactivity or interference between Human KIM-1and analogues was observed.
Typical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
| Concentration(ng/mL) | O.D | Average | Corrected |
|---|---|---|---|
| 10 | 2.3752.409 | 2.392 | 2.331 |
| 5 | 1.5911.647 | 1.619 | 1.558 |
| 2.5 | 0.9550.929 | 0.942 | 0.881 |
| 1.25 | 0.4460.468 | 0.457 | 0.396 |
| 0.63 | 0.2610.237 | 0.249 | 0.188 |
| 0.31 | 0.1680.154 | 0.161 | 0.1 |
| 0.16 | 0.1070.121 | 0.114 | 0.053 |
| 0 | 0.0570.065 | 0.061 | -- |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human KIM-1were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human KIM-1were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.50 | 1.20 | 4.90 | 0.50 | 1.10 | 4.90 |
| Standard deviation | 0.03 | 0.06 | 0.23 | 0.03 | 0.06 | 0.20 |
| C V (%) | 6.00 | 5.00 | 4.69 | 6.00 | 5.45 | 4.08 |
Recovery
The recovery of Human KIM-1spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum (n=5) | 91-104 | 97 |
| EDTA plasma (n=5) | 91-107 | 98 |
| Cell culture media (n=5) | 95-108 | 102 |
Linearity
Samples were spiked with high concentrations of Human KIM-1and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 90-105 | 95-110 | 84-98 |
| Average (%) | 96 | 103 | 91 | |
| 1:4 | Range (%) | 101-116 | 84-96 | 91-107 |
| Average (%) | 107 | 89 | 98 | |
| 1:8 | Range (%) | 101-117 | 87-102 | 97-110 |
| Average (%) | 107 | 93 | 103 | |
| 1:16 | Range (%) | 100-115 | 82-94 | 96-113 |
| Average (%) | 108 | 87 | 103 |
Kit Components & Storage
An unopened kit can be stored at 4"C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 8 wells X12 strips | -20"C, 6 months |
| Reference Standard | 2 vials | |
| Concentrated Biotinylated Detection Ab (100X) | 1 vial, 120 uL | |
| Concentrated HRP Conjugate (100X) | 1 vial, 120 uL | -20"C(shading light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 4"C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
| HRP Conjugate Diluent | 1 vial, 14 mL | |
| Concentrated Wash Buffer (25X) | 1 vial, 30 mL | |
| Substrate Reagent | 1 vial, 10 mL | 4"C(shading light) |
| Stop Solution | 1 vial, 10 mL | 4"C |
| Plate Sealer | 5 pieces | |
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
Human KIM-1 (Kidney Injury Molecule 1) ELISA Kit (HUES01453) Assay procedure
- 1. Set standard, test sample and control (zero) wells on the pre-coated plate and record theirpositions. It is recommended to measure each standard and sample in duplicate. Note: addall solutions to the bottom of the plate wells while avoiding contact with the well walls. Ensuresolutions do not foam when adding to the wells.
- 2. Aliquot 100µl of standard solutions into the standard wells.
- 3. Add 100µl of Sample / Standard dilution buffer into the control (zero) well.
- 4. Add 100µl of properly diluted sample (serum, plasma, tissue homogenates and otherbiological fluids) into test sample wells.
- 5. Cover the plate with the sealer provided in the kit and incubate for 90 min at 37°C.
- 6. Aspirate the liquid from each well, do not wash. Immediately add 100µL of BiotinylatedDetection Ab working solution to each well. Cover the plate with a plate seal and gently mix.Incubate for 1 hour at 37°C.
- 7. Aspirate or decant the solution from the plate and add 350µL of wash buffer to each welland incubate for 1-2 minutes at room temperature. Aspirate the solution from each well andclap the plate on absorbent filter paper to dry. Repeat this process 3 times. Note: a microplatewasher can be used in this step and other wash steps.
- 8. Add 100µL of HRP Conjugate working solution to each well. Cover with a plate seal andincubate for 30 min at 37°C.
- 9. Aspirate or decant the solution from each well. Repeat the wash process for five times asconducted in step 7.
- 10. Add 90µL of Substrate Reagent to each well. Cover with a new plate seal and incubate forapproximately 15 min at 37°C. Protect the plate from light. Note: the reaction time can beshortened or extended according to the actual color change, but not by more than 30min.
- 11. Add 50 µL of Stop Solution to each well. Note: Adding the stop solution should be done inthe same order as the substrate solution.
- 12. Determine the optical density (OD value) of each well immediately with a microplate readerset at 450 nm.
Human KIM-1 (Kidney Injury Molecule 1) ELISA Kit (HUES01453) Protein Information
| UniProt Protein Function: | TIM-1: May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. Belongs to the immunoglobulin superfamily. TIM family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 5q33.2 Cellular Component: integral to membrane Molecular Function:viral receptor activity Biological Process: entry of virus into host cell Disease: Ige Responsiveness, Atopic |
| NCBI Summary: | The protein encoded by this gene is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. The encoded protein may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010] |
| UniProt Code: | Q96D42 |
| NCBI GenInfo Identifier: | 73919877 |
| NCBI Gene ID: | 26762 |
| NCBI Accession: | Q96D42.2 |
| UniProt Secondary Accession: | Q96D42,O43656, |
| UniProt Related Accession: | Q96D42 |
| Molecular Weight: | 364 |
| NCBI Full Name: | Hepatitis A virus cellular receptor 1 |
| NCBI Synonym Full Names: | hepatitis A virus cellular receptor 1 |
| NCBI Official Symbol: | HAVCR1 |
| NCBI Official Synonym Symbols: | TIM; KIM1; TIM1; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1 |
| NCBI Protein Information: | hepatitis A virus cellular receptor 1; kidney injury molecule 1; T-cell membrane protein 1; T-cell immunoglobulin mucin receptor 1; T-cell immunoglobulin mucin family member 1; T cell immunoglobin domain and mucin domain protein 1 |
| UniProt Protein Name: | Hepatitis A virus cellular receptor 1 |
| UniProt Synonym Protein Names: | Kidney injury molecule 1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; T-cell immunoglobulin mucin receptor 1; TIM; TIM-1; T-cell membrane protein 1 |
| UniProt Gene Name: | HAVCR1 |
| UniProt Entry Name: | HAVR1_HUMAN |
Additional Information
Reactivity: | Human |
ELISA Type: | Sandwich |
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